Recombinant:Article Title: Multiomic analysis of ART-interruption cohorts identifies cell-extrinsic and -intrinsic mechanisms driving lymphocyte-mediated control of HIV rebound.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples PBMCs from people with HIV ACTG A5345, REDUC, TEACH, CLEAR, N/A Tonsils from people without HIV Cooperative Human Tissue Network (CHTN) N/A PBMCs from people without HIV https://www.vitalant.org N/A Chemicals, peptides, and recombinant proteins Human TruStain FcXTM Fc Block BioLegend Cat# 422302; RRID: AB_2818986 Fetal Bovine Serum VWR Cat# 97068-085 RPMI-1640 medium Corning Cat# 10-040-CM Lymphoprep density gradient medium StemCell Technologies Cat# 07851 Penicillin-Streptomycin-Glutamine Thermo Fisher Scientific Cat# 10378016 Metal Contaminant-Free PBS Rockland Cat# MB-008 Normal Mouse Serum Thermo Fisher Scientific Cat# 10410 Normal Rat Serum Thermo Fisher Scientific Cat# 10710C Human Serum from Male AB Plasma Sigma-Aldrich Cat# H4522 Iridium Interchelator Solution Standard Biotools Cat# 201192B EQ Four Element Calibration Beads Standard Biotools Cat# 201078 BD CompBeads BD Biosciences Cat# 555843 16% Paraformaldehyde Electron Microscopy Sciences Cat# 15710 Antibiotic-Antimycotic Solution, 100X Corning Cat# 30-004-CI DNase I Sigma Cat# 11284932001 Phorbol 12-myristate 13-acetate (PMA) Selleckchemor Cat# S7791 Torin Selleckchem Cat# S2827 SMAP-2 MedChemExpress HY Cat# 120272 Metformin Sigma-Aldrich Cat# PHR1084 Puromycin Thermo Fisher Cat# A1113803 Doxycycline StemCell Technologies Cat# 72742 Critical commercial assays MaxPar X8 Antibody Labeling Kit Standard Biotools Cat# 201169B Cell-ID 20-Plex Pd Barcoding Kit Standard Biotools Cat# 205060 Foxp3 Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 MaxPar Cell Acquisition Solution Standard Biotools Cat# 201240 MaxPar PBS Standard Biotools Cat# 201058 MaxPar Cell Staining Buffer Standard Biotools Cat# 201068 LentiX p24Gag Rapid Titer Kit Takara Bio Inc Cat# 632200 Zombie AquaTM Fixable Viability Kit BioLegend Cat# 423102; NULISAseq Inflammation Panel 250 Alamar Biosciences https://alamarbio.com/products-and- services/nulisa-inflammation-panel/ EasySepTM Human CD4 + T Cell Isolation Kit StemCell Technologies Cat# 100-0696 NEB Next Ultra II RNA Library Prep Kit for Illumina New England Biolabs Cat# E7770 Deposited data Raw CyTOF datasets This paper http://datadryad.org/share/GGND55gKi_ JGqv-48c2uKc3yxMwKI7rioHbwm9FKRzY Raw RNAseq data This paper Gene Expression Omnibus (GEO) database under accession number GSE290636 Recombinant DNA pHIV-F4-HSA (pNL-HSA.6ATRiC109FPB4.ecto) Cavrois et al. (PMID 28746881) N/A (Continued on next page) e3 Immunity 59, 1–19.e1–e9, May 12, 2026 ..
Article Title: Minimally invasive diagnosis of precancerous cervical lesions using single-cell peripheral immune atlas.
Article Snippet: .. Chemicals, peptides, and recombinant proteins Ficoll-PaqueTM PLUS GE Healthcare Cat# 17-1440-03 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# V900933-1KG Fc Receptor Blocking Solution In House N/A BioStab Antibody Stabilizer MERCK Cat# 55514 Maxpar® Antibody Labeling Kit Standard BioTools N/A Maxpar® Fix and Perm Buffer Standard BioTools Cat# 201067 Cell-IDTM Cisplatin-194Pt Standard BioTools Cat# 201194 Cell-IDTM Intercalator-Ir Standard BioTools Cat# 201192B EQ Four Element Calibration Beads Standard BioTools Cat# 201078 Tuning Solution Standard BioTools Cat# 201072 Washing Solution Standard BioTools Cat# 201070 Palladium isotopes TRACE N/A Deposited data Mass cytometry data of peripheral immune cells This paper OMIX database: OMIX009788; https://ngdc.cncb.ac.cn/omix/ Experimental models: Organisms/strains Peripheral blood This paper Women’s Hospital, Zhejiang University School of Medicine Software and algorithms FlowJo v10.0.7 BD Biosciences RRID:SCR_008520; https://www.flowjo.com/ R v3.6.1 R Core Team (2019) RRID:SCR_001905; https://www.R-project.org/ Python (3.10) Python Software Foundation RRID:SCR_008394; https://www.python.org/ e2 Cell Reports Medicine 6, 102149, June 17, 2025 Article ll OPEN ACCESS squamous intraepithelial lesion (HSIL), atypical glandular cells (AGC), adenocarcinoma in situ (AIS), and cancer cells. ..
Blocking Assay:Article Title: Multiomic analysis of ART-interruption cohorts identifies cell-extrinsic and -intrinsic mechanisms driving lymphocyte-mediated control of HIV rebound.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples PBMCs from people with HIV ACTG A5345, REDUC, TEACH, CLEAR, N/A Tonsils from people without HIV Cooperative Human Tissue Network (CHTN) N/A PBMCs from people without HIV https://www.vitalant.org N/A Chemicals, peptides, and recombinant proteins Human TruStain FcXTM Fc Block BioLegend Cat# 422302; RRID: AB_2818986 Fetal Bovine Serum VWR Cat# 97068-085 RPMI-1640 medium Corning Cat# 10-040-CM Lymphoprep density gradient medium StemCell Technologies Cat# 07851 Penicillin-Streptomycin-Glutamine Thermo Fisher Scientific Cat# 10378016 Metal Contaminant-Free PBS Rockland Cat# MB-008 Normal Mouse Serum Thermo Fisher Scientific Cat# 10410 Normal Rat Serum Thermo Fisher Scientific Cat# 10710C Human Serum from Male AB Plasma Sigma-Aldrich Cat# H4522 Iridium Interchelator Solution Standard Biotools Cat# 201192B EQ Four Element Calibration Beads Standard Biotools Cat# 201078 BD CompBeads BD Biosciences Cat# 555843 16% Paraformaldehyde Electron Microscopy Sciences Cat# 15710 Antibiotic-Antimycotic Solution, 100X Corning Cat# 30-004-CI DNase I Sigma Cat# 11284932001 Phorbol 12-myristate 13-acetate (PMA) Selleckchemor Cat# S7791 Torin Selleckchem Cat# S2827 SMAP-2 MedChemExpress HY Cat# 120272 Metformin Sigma-Aldrich Cat# PHR1084 Puromycin Thermo Fisher Cat# A1113803 Doxycycline StemCell Technologies Cat# 72742 Critical commercial assays MaxPar X8 Antibody Labeling Kit Standard Biotools Cat# 201169B Cell-ID 20-Plex Pd Barcoding Kit Standard Biotools Cat# 205060 Foxp3 Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 MaxPar Cell Acquisition Solution Standard Biotools Cat# 201240 MaxPar PBS Standard Biotools Cat# 201058 MaxPar Cell Staining Buffer Standard Biotools Cat# 201068 LentiX p24Gag Rapid Titer Kit Takara Bio Inc Cat# 632200 Zombie AquaTM Fixable Viability Kit BioLegend Cat# 423102; NULISAseq Inflammation Panel 250 Alamar Biosciences https://alamarbio.com/products-and- services/nulisa-inflammation-panel/ EasySepTM Human CD4 + T Cell Isolation Kit StemCell Technologies Cat# 100-0696 NEB Next Ultra II RNA Library Prep Kit for Illumina New England Biolabs Cat# E7770 Deposited data Raw CyTOF datasets This paper http://datadryad.org/share/GGND55gKi_ JGqv-48c2uKc3yxMwKI7rioHbwm9FKRzY Raw RNAseq data This paper Gene Expression Omnibus (GEO) database under accession number GSE290636 Recombinant DNA pHIV-F4-HSA (pNL-HSA.6ATRiC109FPB4.ecto) Cavrois et al. (PMID 28746881) N/A (Continued on next page) e3 Immunity 59, 1–19.e1–e9, May 12, 2026 ..
Article Title: Minimally invasive diagnosis of precancerous cervical lesions using single-cell peripheral immune atlas.
Article Snippet: .. Chemicals, peptides, and recombinant proteins Ficoll-PaqueTM PLUS GE Healthcare Cat# 17-1440-03 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# V900933-1KG Fc Receptor Blocking Solution In House N/A BioStab Antibody Stabilizer MERCK Cat# 55514 Maxpar® Antibody Labeling Kit Standard BioTools N/A Maxpar® Fix and Perm Buffer Standard BioTools Cat# 201067 Cell-IDTM Cisplatin-194Pt Standard BioTools Cat# 201194 Cell-IDTM Intercalator-Ir Standard BioTools Cat# 201192B EQ Four Element Calibration Beads Standard BioTools Cat# 201078 Tuning Solution Standard BioTools Cat# 201072 Washing Solution Standard BioTools Cat# 201070 Palladium isotopes TRACE N/A Deposited data Mass cytometry data of peripheral immune cells This paper OMIX database: OMIX009788; https://ngdc.cncb.ac.cn/omix/ Experimental models: Organisms/strains Peripheral blood This paper Women’s Hospital, Zhejiang University School of Medicine Software and algorithms FlowJo v10.0.7 BD Biosciences RRID:SCR_008520; https://www.flowjo.com/ R v3.6.1 R Core Team (2019) RRID:SCR_001905; https://www.R-project.org/ Python (3.10) Python Software Foundation RRID:SCR_008394; https://www.python.org/ e2 Cell Reports Medicine 6, 102149, June 17, 2025 Article ll OPEN ACCESS squamous intraepithelial lesion (HSIL), atypical glandular cells (AGC), adenocarcinoma in situ (AIS), and cancer cells. ..
Clinical Proteomics:Article Title: Multiomic analysis of ART-interruption cohorts identifies cell-extrinsic and -intrinsic mechanisms driving lymphocyte-mediated control of HIV rebound.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples PBMCs from people with HIV ACTG A5345, REDUC, TEACH, CLEAR, N/A Tonsils from people without HIV Cooperative Human Tissue Network (CHTN) N/A PBMCs from people without HIV https://www.vitalant.org N/A Chemicals, peptides, and recombinant proteins Human TruStain FcXTM Fc Block BioLegend Cat# 422302; RRID: AB_2818986 Fetal Bovine Serum VWR Cat# 97068-085 RPMI-1640 medium Corning Cat# 10-040-CM Lymphoprep density gradient medium StemCell Technologies Cat# 07851 Penicillin-Streptomycin-Glutamine Thermo Fisher Scientific Cat# 10378016 Metal Contaminant-Free PBS Rockland Cat# MB-008 Normal Mouse Serum Thermo Fisher Scientific Cat# 10410 Normal Rat Serum Thermo Fisher Scientific Cat# 10710C Human Serum from Male AB Plasma Sigma-Aldrich Cat# H4522 Iridium Interchelator Solution Standard Biotools Cat# 201192B EQ Four Element Calibration Beads Standard Biotools Cat# 201078 BD CompBeads BD Biosciences Cat# 555843 16% Paraformaldehyde Electron Microscopy Sciences Cat# 15710 Antibiotic-Antimycotic Solution, 100X Corning Cat# 30-004-CI DNase I Sigma Cat# 11284932001 Phorbol 12-myristate 13-acetate (PMA) Selleckchemor Cat# S7791 Torin Selleckchem Cat# S2827 SMAP-2 MedChemExpress HY Cat# 120272 Metformin Sigma-Aldrich Cat# PHR1084 Puromycin Thermo Fisher Cat# A1113803 Doxycycline StemCell Technologies Cat# 72742 Critical commercial assays MaxPar X8 Antibody Labeling Kit Standard Biotools Cat# 201169B Cell-ID 20-Plex Pd Barcoding Kit Standard Biotools Cat# 205060 Foxp3 Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 MaxPar Cell Acquisition Solution Standard Biotools Cat# 201240 MaxPar PBS Standard Biotools Cat# 201058 MaxPar Cell Staining Buffer Standard Biotools Cat# 201068 LentiX p24Gag Rapid Titer Kit Takara Bio Inc Cat# 632200 Zombie AquaTM Fixable Viability Kit BioLegend Cat# 423102; NULISAseq Inflammation Panel 250 Alamar Biosciences https://alamarbio.com/products-and- services/nulisa-inflammation-panel/ EasySepTM Human CD4 + T Cell Isolation Kit StemCell Technologies Cat# 100-0696 NEB Next Ultra II RNA Library Prep Kit for Illumina New England Biolabs Cat# E7770 Deposited data Raw CyTOF datasets This paper http://datadryad.org/share/GGND55gKi_ JGqv-48c2uKc3yxMwKI7rioHbwm9FKRzY Raw RNAseq data This paper Gene Expression Omnibus (GEO) database under accession number GSE290636 Recombinant DNA pHIV-F4-HSA (pNL-HSA.6ATRiC109FPB4.ecto) Cavrois et al. (PMID 28746881) N/A (Continued on next page) e3 Immunity 59, 1–19.e1–e9, May 12, 2026 ..
Electron Microscopy:Article Title: Multiomic analysis of ART-interruption cohorts identifies cell-extrinsic and -intrinsic mechanisms driving lymphocyte-mediated control of HIV rebound.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples PBMCs from people with HIV ACTG A5345, REDUC, TEACH, CLEAR, N/A Tonsils from people without HIV Cooperative Human Tissue Network (CHTN) N/A PBMCs from people without HIV https://www.vitalant.org N/A Chemicals, peptides, and recombinant proteins Human TruStain FcXTM Fc Block BioLegend Cat# 422302; RRID: AB_2818986 Fetal Bovine Serum VWR Cat# 97068-085 RPMI-1640 medium Corning Cat# 10-040-CM Lymphoprep density gradient medium StemCell Technologies Cat# 07851 Penicillin-Streptomycin-Glutamine Thermo Fisher Scientific Cat# 10378016 Metal Contaminant-Free PBS Rockland Cat# MB-008 Normal Mouse Serum Thermo Fisher Scientific Cat# 10410 Normal Rat Serum Thermo Fisher Scientific Cat# 10710C Human Serum from Male AB Plasma Sigma-Aldrich Cat# H4522 Iridium Interchelator Solution Standard Biotools Cat# 201192B EQ Four Element Calibration Beads Standard Biotools Cat# 201078 BD CompBeads BD Biosciences Cat# 555843 16% Paraformaldehyde Electron Microscopy Sciences Cat# 15710 Antibiotic-Antimycotic Solution, 100X Corning Cat# 30-004-CI DNase I Sigma Cat# 11284932001 Phorbol 12-myristate 13-acetate (PMA) Selleckchemor Cat# S7791 Torin Selleckchem Cat# S2827 SMAP-2 MedChemExpress HY Cat# 120272 Metformin Sigma-Aldrich Cat# PHR1084 Puromycin Thermo Fisher Cat# A1113803 Doxycycline StemCell Technologies Cat# 72742 Critical commercial assays MaxPar X8 Antibody Labeling Kit Standard Biotools Cat# 201169B Cell-ID 20-Plex Pd Barcoding Kit Standard Biotools Cat# 205060 Foxp3 Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 MaxPar Cell Acquisition Solution Standard Biotools Cat# 201240 MaxPar PBS Standard Biotools Cat# 201058 MaxPar Cell Staining Buffer Standard Biotools Cat# 201068 LentiX p24Gag Rapid Titer Kit Takara Bio Inc Cat# 632200 Zombie AquaTM Fixable Viability Kit BioLegend Cat# 423102; NULISAseq Inflammation Panel 250 Alamar Biosciences https://alamarbio.com/products-and- services/nulisa-inflammation-panel/ EasySepTM Human CD4 + T Cell Isolation Kit StemCell Technologies Cat# 100-0696 NEB Next Ultra II RNA Library Prep Kit for Illumina New England Biolabs Cat# E7770 Deposited data Raw CyTOF datasets This paper http://datadryad.org/share/GGND55gKi_ JGqv-48c2uKc3yxMwKI7rioHbwm9FKRzY Raw RNAseq data This paper Gene Expression Omnibus (GEO) database under accession number GSE290636 Recombinant DNA pHIV-F4-HSA (pNL-HSA.6ATRiC109FPB4.ecto) Cavrois et al. (PMID 28746881) N/A (Continued on next page) e3 Immunity 59, 1–19.e1–e9, May 12, 2026 ..
Antibody Labeling:Article Title: Multiomic analysis of ART-interruption cohorts identifies cell-extrinsic and -intrinsic mechanisms driving lymphocyte-mediated control of HIV rebound.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples PBMCs from people with HIV ACTG A5345, REDUC, TEACH, CLEAR, N/A Tonsils from people without HIV Cooperative Human Tissue Network (CHTN) N/A PBMCs from people without HIV https://www.vitalant.org N/A Chemicals, peptides, and recombinant proteins Human TruStain FcXTM Fc Block BioLegend Cat# 422302; RRID: AB_2818986 Fetal Bovine Serum VWR Cat# 97068-085 RPMI-1640 medium Corning Cat# 10-040-CM Lymphoprep density gradient medium StemCell Technologies Cat# 07851 Penicillin-Streptomycin-Glutamine Thermo Fisher Scientific Cat# 10378016 Metal Contaminant-Free PBS Rockland Cat# MB-008 Normal Mouse Serum Thermo Fisher Scientific Cat# 10410 Normal Rat Serum Thermo Fisher Scientific Cat# 10710C Human Serum from Male AB Plasma Sigma-Aldrich Cat# H4522 Iridium Interchelator Solution Standard Biotools Cat# 201192B EQ Four Element Calibration Beads Standard Biotools Cat# 201078 BD CompBeads BD Biosciences Cat# 555843 16% Paraformaldehyde Electron Microscopy Sciences Cat# 15710 Antibiotic-Antimycotic Solution, 100X Corning Cat# 30-004-CI DNase I Sigma Cat# 11284932001 Phorbol 12-myristate 13-acetate (PMA) Selleckchemor Cat# S7791 Torin Selleckchem Cat# S2827 SMAP-2 MedChemExpress HY Cat# 120272 Metformin Sigma-Aldrich Cat# PHR1084 Puromycin Thermo Fisher Cat# A1113803 Doxycycline StemCell Technologies Cat# 72742 Critical commercial assays MaxPar X8 Antibody Labeling Kit Standard Biotools Cat# 201169B Cell-ID 20-Plex Pd Barcoding Kit Standard Biotools Cat# 205060 Foxp3 Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 MaxPar Cell Acquisition Solution Standard Biotools Cat# 201240 MaxPar PBS Standard Biotools Cat# 201058 MaxPar Cell Staining Buffer Standard Biotools Cat# 201068 LentiX p24Gag Rapid Titer Kit Takara Bio Inc Cat# 632200 Zombie AquaTM Fixable Viability Kit BioLegend Cat# 423102; NULISAseq Inflammation Panel 250 Alamar Biosciences https://alamarbio.com/products-and- services/nulisa-inflammation-panel/ EasySepTM Human CD4 + T Cell Isolation Kit StemCell Technologies Cat# 100-0696 NEB Next Ultra II RNA Library Prep Kit for Illumina New England Biolabs Cat# E7770 Deposited data Raw CyTOF datasets This paper http://datadryad.org/share/GGND55gKi_ JGqv-48c2uKc3yxMwKI7rioHbwm9FKRzY Raw RNAseq data This paper Gene Expression Omnibus (GEO) database under accession number GSE290636 Recombinant DNA pHIV-F4-HSA (pNL-HSA.6ATRiC109FPB4.ecto) Cavrois et al. (PMID 28746881) N/A (Continued on next page) e3 Immunity 59, 1–19.e1–e9, May 12, 2026 ..
Article Title: Minimally invasive diagnosis of precancerous cervical lesions using single-cell peripheral immune atlas.
Article Snippet: .. Chemicals, peptides, and recombinant proteins Ficoll-PaqueTM PLUS GE Healthcare Cat# 17-1440-03 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# V900933-1KG Fc Receptor Blocking Solution In House N/A BioStab Antibody Stabilizer MERCK Cat# 55514 Maxpar® Antibody Labeling Kit Standard BioTools N/A Maxpar® Fix and Perm Buffer Standard BioTools Cat# 201067 Cell-IDTM Cisplatin-194Pt Standard BioTools Cat# 201194 Cell-IDTM Intercalator-Ir Standard BioTools Cat# 201192B EQ Four Element Calibration Beads Standard BioTools Cat# 201078 Tuning Solution Standard BioTools Cat# 201072 Washing Solution Standard BioTools Cat# 201070 Palladium isotopes TRACE N/A Deposited data Mass cytometry data of peripheral immune cells This paper OMIX database: OMIX009788; https://ngdc.cncb.ac.cn/omix/ Experimental models: Organisms/strains Peripheral blood This paper Women’s Hospital, Zhejiang University School of Medicine Software and algorithms FlowJo v10.0.7 BD Biosciences RRID:SCR_008520; https://www.flowjo.com/ R v3.6.1 R Core Team (2019) RRID:SCR_001905; https://www.R-project.org/ Python (3.10) Python Software Foundation RRID:SCR_008394; https://www.python.org/ e2 Cell Reports Medicine 6, 102149, June 17, 2025 Article ll OPEN ACCESS squamous intraepithelial lesion (HSIL), atypical glandular cells (AGC), adenocarcinoma in situ (AIS), and cancer cells. ..
Staining:Article Title: Multiomic analysis of ART-interruption cohorts identifies cell-extrinsic and -intrinsic mechanisms driving lymphocyte-mediated control of HIV rebound.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples PBMCs from people with HIV ACTG A5345, REDUC, TEACH, CLEAR, N/A Tonsils from people without HIV Cooperative Human Tissue Network (CHTN) N/A PBMCs from people without HIV https://www.vitalant.org N/A Chemicals, peptides, and recombinant proteins Human TruStain FcXTM Fc Block BioLegend Cat# 422302; RRID: AB_2818986 Fetal Bovine Serum VWR Cat# 97068-085 RPMI-1640 medium Corning Cat# 10-040-CM Lymphoprep density gradient medium StemCell Technologies Cat# 07851 Penicillin-Streptomycin-Glutamine Thermo Fisher Scientific Cat# 10378016 Metal Contaminant-Free PBS Rockland Cat# MB-008 Normal Mouse Serum Thermo Fisher Scientific Cat# 10410 Normal Rat Serum Thermo Fisher Scientific Cat# 10710C Human Serum from Male AB Plasma Sigma-Aldrich Cat# H4522 Iridium Interchelator Solution Standard Biotools Cat# 201192B EQ Four Element Calibration Beads Standard Biotools Cat# 201078 BD CompBeads BD Biosciences Cat# 555843 16% Paraformaldehyde Electron Microscopy Sciences Cat# 15710 Antibiotic-Antimycotic Solution, 100X Corning Cat# 30-004-CI DNase I Sigma Cat# 11284932001 Phorbol 12-myristate 13-acetate (PMA) Selleckchemor Cat# S7791 Torin Selleckchem Cat# S2827 SMAP-2 MedChemExpress HY Cat# 120272 Metformin Sigma-Aldrich Cat# PHR1084 Puromycin Thermo Fisher Cat# A1113803 Doxycycline StemCell Technologies Cat# 72742 Critical commercial assays MaxPar X8 Antibody Labeling Kit Standard Biotools Cat# 201169B Cell-ID 20-Plex Pd Barcoding Kit Standard Biotools Cat# 205060 Foxp3 Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 MaxPar Cell Acquisition Solution Standard Biotools Cat# 201240 MaxPar PBS Standard Biotools Cat# 201058 MaxPar Cell Staining Buffer Standard Biotools Cat# 201068 LentiX p24Gag Rapid Titer Kit Takara Bio Inc Cat# 632200 Zombie AquaTM Fixable Viability Kit BioLegend Cat# 423102; NULISAseq Inflammation Panel 250 Alamar Biosciences https://alamarbio.com/products-and- services/nulisa-inflammation-panel/ EasySepTM Human CD4 + T Cell Isolation Kit StemCell Technologies Cat# 100-0696 NEB Next Ultra II RNA Library Prep Kit for Illumina New England Biolabs Cat# E7770 Deposited data Raw CyTOF datasets This paper http://datadryad.org/share/GGND55gKi_ JGqv-48c2uKc3yxMwKI7rioHbwm9FKRzY Raw RNAseq data This paper Gene Expression Omnibus (GEO) database under accession number GSE290636 Recombinant DNA pHIV-F4-HSA (pNL-HSA.6ATRiC109FPB4.ecto) Cavrois et al. (PMID 28746881) N/A (Continued on next page) e3 Immunity 59, 1–19.e1–e9, May 12, 2026 ..
Cell Isolation:Article Title: Multiomic analysis of ART-interruption cohorts identifies cell-extrinsic and -intrinsic mechanisms driving lymphocyte-mediated control of HIV rebound.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples PBMCs from people with HIV ACTG A5345, REDUC, TEACH, CLEAR, N/A Tonsils from people without HIV Cooperative Human Tissue Network (CHTN) N/A PBMCs from people without HIV https://www.vitalant.org N/A Chemicals, peptides, and recombinant proteins Human TruStain FcXTM Fc Block BioLegend Cat# 422302; RRID: AB_2818986 Fetal Bovine Serum VWR Cat# 97068-085 RPMI-1640 medium Corning Cat# 10-040-CM Lymphoprep density gradient medium StemCell Technologies Cat# 07851 Penicillin-Streptomycin-Glutamine Thermo Fisher Scientific Cat# 10378016 Metal Contaminant-Free PBS Rockland Cat# MB-008 Normal Mouse Serum Thermo Fisher Scientific Cat# 10410 Normal Rat Serum Thermo Fisher Scientific Cat# 10710C Human Serum from Male AB Plasma Sigma-Aldrich Cat# H4522 Iridium Interchelator Solution Standard Biotools Cat# 201192B EQ Four Element Calibration Beads Standard Biotools Cat# 201078 BD CompBeads BD Biosciences Cat# 555843 16% Paraformaldehyde Electron Microscopy Sciences Cat# 15710 Antibiotic-Antimycotic Solution, 100X Corning Cat# 30-004-CI DNase I Sigma Cat# 11284932001 Phorbol 12-myristate 13-acetate (PMA) Selleckchemor Cat# S7791 Torin Selleckchem Cat# S2827 SMAP-2 MedChemExpress HY Cat# 120272 Metformin Sigma-Aldrich Cat# PHR1084 Puromycin Thermo Fisher Cat# A1113803 Doxycycline StemCell Technologies Cat# 72742 Critical commercial assays MaxPar X8 Antibody Labeling Kit Standard Biotools Cat# 201169B Cell-ID 20-Plex Pd Barcoding Kit Standard Biotools Cat# 205060 Foxp3 Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 MaxPar Cell Acquisition Solution Standard Biotools Cat# 201240 MaxPar PBS Standard Biotools Cat# 201058 MaxPar Cell Staining Buffer Standard Biotools Cat# 201068 LentiX p24Gag Rapid Titer Kit Takara Bio Inc Cat# 632200 Zombie AquaTM Fixable Viability Kit BioLegend Cat# 423102; NULISAseq Inflammation Panel 250 Alamar Biosciences https://alamarbio.com/products-and- services/nulisa-inflammation-panel/ EasySepTM Human CD4 + T Cell Isolation Kit StemCell Technologies Cat# 100-0696 NEB Next Ultra II RNA Library Prep Kit for Illumina New England Biolabs Cat# E7770 Deposited data Raw CyTOF datasets This paper http://datadryad.org/share/GGND55gKi_ JGqv-48c2uKc3yxMwKI7rioHbwm9FKRzY Raw RNAseq data This paper Gene Expression Omnibus (GEO) database under accession number GSE290636 Recombinant DNA pHIV-F4-HSA (pNL-HSA.6ATRiC109FPB4.ecto) Cavrois et al. (PMID 28746881) N/A (Continued on next page) e3 Immunity 59, 1–19.e1–e9, May 12, 2026 ..
RNA sequencing:Article Title: Multiomic analysis of ART-interruption cohorts identifies cell-extrinsic and -intrinsic mechanisms driving lymphocyte-mediated control of HIV rebound.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples PBMCs from people with HIV ACTG A5345, REDUC, TEACH, CLEAR, N/A Tonsils from people without HIV Cooperative Human Tissue Network (CHTN) N/A PBMCs from people without HIV https://www.vitalant.org N/A Chemicals, peptides, and recombinant proteins Human TruStain FcXTM Fc Block BioLegend Cat# 422302; RRID: AB_2818986 Fetal Bovine Serum VWR Cat# 97068-085 RPMI-1640 medium Corning Cat# 10-040-CM Lymphoprep density gradient medium StemCell Technologies Cat# 07851 Penicillin-Streptomycin-Glutamine Thermo Fisher Scientific Cat# 10378016 Metal Contaminant-Free PBS Rockland Cat# MB-008 Normal Mouse Serum Thermo Fisher Scientific Cat# 10410 Normal Rat Serum Thermo Fisher Scientific Cat# 10710C Human Serum from Male AB Plasma Sigma-Aldrich Cat# H4522 Iridium Interchelator Solution Standard Biotools Cat# 201192B EQ Four Element Calibration Beads Standard Biotools Cat# 201078 BD CompBeads BD Biosciences Cat# 555843 16% Paraformaldehyde Electron Microscopy Sciences Cat# 15710 Antibiotic-Antimycotic Solution, 100X Corning Cat# 30-004-CI DNase I Sigma Cat# 11284932001 Phorbol 12-myristate 13-acetate (PMA) Selleckchemor Cat# S7791 Torin Selleckchem Cat# S2827 SMAP-2 MedChemExpress HY Cat# 120272 Metformin Sigma-Aldrich Cat# PHR1084 Puromycin Thermo Fisher Cat# A1113803 Doxycycline StemCell Technologies Cat# 72742 Critical commercial assays MaxPar X8 Antibody Labeling Kit Standard Biotools Cat# 201169B Cell-ID 20-Plex Pd Barcoding Kit Standard Biotools Cat# 205060 Foxp3 Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 MaxPar Cell Acquisition Solution Standard Biotools Cat# 201240 MaxPar PBS Standard Biotools Cat# 201058 MaxPar Cell Staining Buffer Standard Biotools Cat# 201068 LentiX p24Gag Rapid Titer Kit Takara Bio Inc Cat# 632200 Zombie AquaTM Fixable Viability Kit BioLegend Cat# 423102; NULISAseq Inflammation Panel 250 Alamar Biosciences https://alamarbio.com/products-and- services/nulisa-inflammation-panel/ EasySepTM Human CD4 + T Cell Isolation Kit StemCell Technologies Cat# 100-0696 NEB Next Ultra II RNA Library Prep Kit for Illumina New England Biolabs Cat# E7770 Deposited data Raw CyTOF datasets This paper http://datadryad.org/share/GGND55gKi_ JGqv-48c2uKc3yxMwKI7rioHbwm9FKRzY Raw RNAseq data This paper Gene Expression Omnibus (GEO) database under accession number GSE290636 Recombinant DNA pHIV-F4-HSA (pNL-HSA.6ATRiC109FPB4.ecto) Cavrois et al. (PMID 28746881) N/A (Continued on next page) e3 Immunity 59, 1–19.e1–e9, May 12, 2026 ..
Gene Expression:Article Title: Multiomic analysis of ART-interruption cohorts identifies cell-extrinsic and -intrinsic mechanisms driving lymphocyte-mediated control of HIV rebound.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples PBMCs from people with HIV ACTG A5345, REDUC, TEACH, CLEAR, N/A Tonsils from people without HIV Cooperative Human Tissue Network (CHTN) N/A PBMCs from people without HIV https://www.vitalant.org N/A Chemicals, peptides, and recombinant proteins Human TruStain FcXTM Fc Block BioLegend Cat# 422302; RRID: AB_2818986 Fetal Bovine Serum VWR Cat# 97068-085 RPMI-1640 medium Corning Cat# 10-040-CM Lymphoprep density gradient medium StemCell Technologies Cat# 07851 Penicillin-Streptomycin-Glutamine Thermo Fisher Scientific Cat# 10378016 Metal Contaminant-Free PBS Rockland Cat# MB-008 Normal Mouse Serum Thermo Fisher Scientific Cat# 10410 Normal Rat Serum Thermo Fisher Scientific Cat# 10710C Human Serum from Male AB Plasma Sigma-Aldrich Cat# H4522 Iridium Interchelator Solution Standard Biotools Cat# 201192B EQ Four Element Calibration Beads Standard Biotools Cat# 201078 BD CompBeads BD Biosciences Cat# 555843 16% Paraformaldehyde Electron Microscopy Sciences Cat# 15710 Antibiotic-Antimycotic Solution, 100X Corning Cat# 30-004-CI DNase I Sigma Cat# 11284932001 Phorbol 12-myristate 13-acetate (PMA) Selleckchemor Cat# S7791 Torin Selleckchem Cat# S2827 SMAP-2 MedChemExpress HY Cat# 120272 Metformin Sigma-Aldrich Cat# PHR1084 Puromycin Thermo Fisher Cat# A1113803 Doxycycline StemCell Technologies Cat# 72742 Critical commercial assays MaxPar X8 Antibody Labeling Kit Standard Biotools Cat# 201169B Cell-ID 20-Plex Pd Barcoding Kit Standard Biotools Cat# 205060 Foxp3 Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 MaxPar Cell Acquisition Solution Standard Biotools Cat# 201240 MaxPar PBS Standard Biotools Cat# 201058 MaxPar Cell Staining Buffer Standard Biotools Cat# 201068 LentiX p24Gag Rapid Titer Kit Takara Bio Inc Cat# 632200 Zombie AquaTM Fixable Viability Kit BioLegend Cat# 423102; NULISAseq Inflammation Panel 250 Alamar Biosciences https://alamarbio.com/products-and- services/nulisa-inflammation-panel/ EasySepTM Human CD4 + T Cell Isolation Kit StemCell Technologies Cat# 100-0696 NEB Next Ultra II RNA Library Prep Kit for Illumina New England Biolabs Cat# E7770 Deposited data Raw CyTOF datasets This paper http://datadryad.org/share/GGND55gKi_ JGqv-48c2uKc3yxMwKI7rioHbwm9FKRzY Raw RNAseq data This paper Gene Expression Omnibus (GEO) database under accession number GSE290636 Recombinant DNA pHIV-F4-HSA (pNL-HSA.6ATRiC109FPB4.ecto) Cavrois et al. (PMID 28746881) N/A (Continued on next page) e3 Immunity 59, 1–19.e1–e9, May 12, 2026 ..
other:Article Title: Anatomical, subset, and HIV-dependent expression of viral sensors and restriction factors.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GraphPad Prism (Version 10.2.0) http://www.graphpad.com/ RRID:SCR_002798 RStudio (2022.07.2 + 576 "Spotted Wakerobin" Release) https://rstudio.com/ RRID:SCR_000432 R Project for Statistical Computing (Version 4.2.1) http://www.r-project.org/ RRID:SCR_001905 CUHIMSR/CytofBatchAdjust Schuyler et al.95; https://github.com/ CUHIMSR/CytofBatchAdjust N/A ggplot2 https://cran.r-project.org/web/ packages/ggplot2/index.html RRID:SCR_014601 Rtsne https://github.com/jkrijthe/Rtsne RRID:SCR_016342 Seurat Hao et al.96; https://satijalab.org/ seurat/get_started.html RRID:SCR_016341 Harmony Korsunsky et al.97; https://github.com/ immunogenomics/harmony RRID:SCR_022206 RandomForest Package in R Breiman98; https://cran.r-project. org/web/packages/randomForest/ RRID:SCR_015718 lme4 Bates et al.99; https://cran.r-project. org/web/packages/lme4/index.html RRID:SCR_015654 emmeans https://CRAN.R-project.org/ package=emmeans RRID:SCR_018734
Mass Cytometry:Article Title: Minimally invasive diagnosis of precancerous cervical lesions using single-cell peripheral immune atlas.
Article Snippet: .. Chemicals, peptides, and recombinant proteins Ficoll-PaqueTM PLUS GE Healthcare Cat# 17-1440-03 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# V900933-1KG Fc Receptor Blocking Solution In House N/A BioStab Antibody Stabilizer MERCK Cat# 55514 Maxpar® Antibody Labeling Kit Standard BioTools N/A Maxpar® Fix and Perm Buffer Standard BioTools Cat# 201067 Cell-IDTM Cisplatin-194Pt Standard BioTools Cat# 201194 Cell-IDTM Intercalator-Ir Standard BioTools Cat# 201192B EQ Four Element Calibration Beads Standard BioTools Cat# 201078 Tuning Solution Standard BioTools Cat# 201072 Washing Solution Standard BioTools Cat# 201070 Palladium isotopes TRACE N/A Deposited data Mass cytometry data of peripheral immune cells This paper OMIX database: OMIX009788; https://ngdc.cncb.ac.cn/omix/ Experimental models: Organisms/strains Peripheral blood This paper Women’s Hospital, Zhejiang University School of Medicine Software and algorithms FlowJo v10.0.7 BD Biosciences RRID:SCR_008520; https://www.flowjo.com/ R v3.6.1 R Core Team (2019) RRID:SCR_001905; https://www.R-project.org/ Python (3.10) Python Software Foundation RRID:SCR_008394; https://www.python.org/ e2 Cell Reports Medicine 6, 102149, June 17, 2025 Article ll OPEN ACCESS squamous intraepithelial lesion (HSIL), atypical glandular cells (AGC), adenocarcinoma in situ (AIS), and cancer cells. ..
Software:Article Title: Minimally invasive diagnosis of precancerous cervical lesions using single-cell peripheral immune atlas.
Article Snippet: .. Chemicals, peptides, and recombinant proteins Ficoll-PaqueTM PLUS GE Healthcare Cat# 17-1440-03 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# V900933-1KG Fc Receptor Blocking Solution In House N/A BioStab Antibody Stabilizer MERCK Cat# 55514 Maxpar® Antibody Labeling Kit Standard BioTools N/A Maxpar® Fix and Perm Buffer Standard BioTools Cat# 201067 Cell-IDTM Cisplatin-194Pt Standard BioTools Cat# 201194 Cell-IDTM Intercalator-Ir Standard BioTools Cat# 201192B EQ Four Element Calibration Beads Standard BioTools Cat# 201078 Tuning Solution Standard BioTools Cat# 201072 Washing Solution Standard BioTools Cat# 201070 Palladium isotopes TRACE N/A Deposited data Mass cytometry data of peripheral immune cells This paper OMIX database: OMIX009788; https://ngdc.cncb.ac.cn/omix/ Experimental models: Organisms/strains Peripheral blood This paper Women’s Hospital, Zhejiang University School of Medicine Software and algorithms FlowJo v10.0.7 BD Biosciences RRID:SCR_008520; https://www.flowjo.com/ R v3.6.1 R Core Team (2019) RRID:SCR_001905; https://www.R-project.org/ Python (3.10) Python Software Foundation RRID:SCR_008394; https://www.python.org/ e2 Cell Reports Medicine 6, 102149, June 17, 2025 Article ll OPEN ACCESS squamous intraepithelial lesion (HSIL), atypical glandular cells (AGC), adenocarcinoma in situ (AIS), and cancer cells. ..
In Situ:Article Title: Minimally invasive diagnosis of precancerous cervical lesions using single-cell peripheral immune atlas.
Article Snippet: .. Chemicals, peptides, and recombinant proteins Ficoll-PaqueTM PLUS GE Healthcare Cat# 17-1440-03 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# V900933-1KG Fc Receptor Blocking Solution In House N/A BioStab Antibody Stabilizer MERCK Cat# 55514 Maxpar® Antibody Labeling Kit Standard BioTools N/A Maxpar® Fix and Perm Buffer Standard BioTools Cat# 201067 Cell-IDTM Cisplatin-194Pt Standard BioTools Cat# 201194 Cell-IDTM Intercalator-Ir Standard BioTools Cat# 201192B EQ Four Element Calibration Beads Standard BioTools Cat# 201078 Tuning Solution Standard BioTools Cat# 201072 Washing Solution Standard BioTools Cat# 201070 Palladium isotopes TRACE N/A Deposited data Mass cytometry data of peripheral immune cells This paper OMIX database: OMIX009788; https://ngdc.cncb.ac.cn/omix/ Experimental models: Organisms/strains Peripheral blood This paper Women’s Hospital, Zhejiang University School of Medicine Software and algorithms FlowJo v10.0.7 BD Biosciences RRID:SCR_008520; https://www.flowjo.com/ R v3.6.1 R Core Team (2019) RRID:SCR_001905; https://www.R-project.org/ Python (3.10) Python Software Foundation RRID:SCR_008394; https://www.python.org/ e2 Cell Reports Medicine 6, 102149, June 17, 2025 Article ll OPEN ACCESS squamous intraepithelial lesion (HSIL), atypical glandular cells (AGC), adenocarcinoma in situ (AIS), and cancer cells. ..
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